The Faculty of Agriculture, Universitas Gadjah Mada (Faperta UGM) organized the Gene Expression Workshop, with the first session focusing on hands-on qPCR (Quantitative Polymerase Chain Reaction) analysis. The event took place on Thursday, April 9, 2026, at the Venture Room, AGLC Building, 6th Floor, and was attended by students and researchers interested in exploring molecular techniques to understand gene expression.
The workshop was designed to provide practical experience in operating the qPCR method, an essential technology in molecular biology research used to accurately measure gene expression levels. Participants not only gained theoretical knowledge but also had the opportunity to conduct direct laboratory experiments, thereby enhancing technical skills while strengthening their understanding of fundamental concepts.
In this session, Prof. Ani Widiastuti, S.P., M.P., Ph.D., as the speaker, emphasized the importance of technical mastery of qPCR as a quantitative method for analyzing gene expression. Participants practiced various stages of analysis, including amplification plots, Ct value interpretation, and melt curve analysis to ensure amplification specificity. She also highlighted several crucial aspects, such as storing reagents under cold conditions, careful pipetting, and preventing air bubbles in PCR tubes.
The workshop not only provided laboratory experience but also reinforced theoretical foundations by referring to recent studies, including Prof. Ani’s work and her team’s research on gene expression related to plant resistance under UV-B exposure.
This workshop supports the achievement of the Sustainable Development Goals (SDGs), particularly SDG 2: Zero Hunger, SDG 3: Good Health and Well-being, SDG 4: Quality Education, SDG 9: Industry, Innovation and Infrastructure, SDG 13: Climate Action, SDG 15: Life on Land, and SDG 17: Partnerships for the Goals.
Author: Beny Nabila Happy Fauziah
Editor: Tantriani
Documentation: Unit Media