After the first session, which focused on hands-on qPCR analysis, the Faculty of Agriculture (Faperta) UGM continued the Gene Expression Workshop with Session 2: Data Processing Using the Livak Method. This session was delivered by Dr. Sc. M. Idris, S.Si., M.Si., who provided an in-depth explanation of the fundamental principles and application of the Livak method in gene expression research.
The Livak Method, or Double Delta Ct Method, is the most widely used mathematical approach in qPCR analysis. Dr. Idris explained that the main objective of this method is to determine the fold-change in gene expression between treated samples and control samples. Participants were guided through the calculation steps, starting from ΔCt (Normalization to the reference gene), ΔΔCt (Comparison with the control), and finally Fold Change (2^-ΔΔCt) as the end result to determine whether a gene is upregulated or downregulated.
In addition, Dr. Idris emphasized the importance of selecting a stable reference gene and ensuring comparable amplification efficiency between the target and reference genes. Participants were also introduced to result interpretation, where a fold change value greater than 1 indicates increased gene expression, while a value less than 1 indicates decreased expression.
This session strengthened participants’ data analysis skills, complementing the laboratory experience from the previous session. By combining practice and theory, the workshop is expected to enhance the capacity of students and researchers in biotechnology research, while also supporting the achievement of the Sustainable Development Goals (SDGs), such as SDG 2: Zero Hunger, SDG 3: Good Health and Well-being, SDG 4: Quality Education, SDG 9: Industry, Innovation and Infrastructure, SDG 13: Climate Action, SDG 15: Life on Land, and SDG 17: Partnerships for the Goals.
Author: Beny Nabila Happy Fauziah
Editor: Tantriani
Documentation: Unit Media Faperta UGM