After the first session, which focused on hands-on qPCR analysis, the Faculty of Agriculture (Faperta) UGM continued the Gene Expression Workshop with Session 2: Data Processing Using the Livak Method. This session was delivered by Dr. Sc. M. Idris, S.Si., M.Si., who provided an in-depth explanation of the fundamental principles and application of the Livak method in gene expression research.

The Livak Method, or Double Delta Ct Method, is the most widely used mathematical approach in qPCR analysis. Dr. Idris explained that the main objective of this method is to determine the fold-change in gene expression between treated samples and control samples. Participants were guided through the calculation steps, starting from ΔCt (Normalization to the reference gene), ΔΔCt (Comparison with the control), and finally Fold Change (2^-ΔΔCt) as the end result to determine whether a gene is upregulated or downregulated. read more

The Faculty of Agriculture, Universitas Gadjah Mada (Faperta UGM) organized the Gene Expression Workshop, with the first session focusing on hands-on qPCR (Quantitative Polymerase Chain Reaction) analysis. The event took place on Thursday, April 9, 2026, at the Venture Room, AGLC Building, 6th Floor, and was attended by students and researchers interested in exploring molecular techniques to understand gene expression.

The workshop was designed to provide practical experience in operating the qPCR method, an essential technology in molecular biology research used to accurately measure gene expression levels. Participants not only gained theoretical knowledge but also had the opportunity to conduct direct laboratory experiments, thereby enhancing technical skills while strengthening their understanding of fundamental concepts. read more

The Faculty of Agriculture, Universitas Gadjah Mada (UGM), successfully organized the Gene Expression Workshop Mastering Real-Time PCR: Understanding Transcriptomic Dynamics Under Climate Change at Venture Room, 6th floor, Agrotropica Learning Center (AGLC) on Thursday, April 9, 2026. This workshop served as an intensive learning platform for academics and practitioners to deepen their understanding of transcriptomic dynamics in addressing the challenges of climate change.

The event commenced with opening remarks from Mgs. Muhammad Prima Putra, S.Pi., M.Sc., Ph.D., Manager of AGLC, and Widhi Dyah Sawitri, S.Si., M.Agr., Ph.D., Deputy Manager of AGLC. They highlighted the importance of cross-disciplinary collaboration in molecular research and introduced the advanced facilities available at AGLC. read more